Every square matrix has eigenvalues and we need to demonstrate that one of those eigenvalues of $A$ has a purely imaginary part.
The solution to $\frac{d}{dt}X=AX$ with $X(t_0)=X_0≠0$ is $e^{At}X_0$.
Plug in the second condition, you get $e^{At_1}X_0=-X_0$ which means $-1$ is an eigenvalue of $e^{t_1A}$.
Let $J$ be a Jordan normal form of $A$ and $A=PJP^{-1}$ where $P$ is some invertible square matrix. Let $J_\lambda$ be the block associated with eigenvalue $\lambda$.
Now $e^{At_1}X_0=-X_0$ is equivalent as $Pe^{t_1J}P^{-1}X_0=-X_0$ and therefore $-1$ is an eigenvalue of $e^{t_1J}$.
Jordon canonical blocks have this nice property: the exponentiation of a Jordon block is an upper triangular matrix with the main diagonal equal to the exponentiation of the eigenvalue.
The same property holds for the whole Jordan form.
In turn, the diagonal elements of upper triangular matrices are the eigenvalues of the matrix.
Therefore, one diagonal element of $e^{t_1J}$ is $-1$. Let the Jordon block where $-1$ is located in be in $e^{t_1J}$ be $J_\lambda$. We then have $e^{t_1\lambda}=-1=e^{i(\frac{\pi}{2}+2n\pi)}$ for $n\in\mathbb{Z}$.
Or, $\lambda=\frac{i\pi}{t_1}(\frac{1}{2}+2n)$, which makes $\lambda$ purely imaginary and non-zero.