As others have said, this quotient is hausdorff iff the relation is closed.
Note that the quotient $\mathbb{R}/\mathbb{Z}$ is in this case (the case where it defines the circle group $S^1$) a group theoretical quotient. The relation is thus
$$\{(a,b)\in \mathbb{R}^2 \mid a-b \in \mathbb{Z}\} = f^{-1}(\mathbb{Z})$$
where
$$f:\mathbb{R}^2 \rightarrow \mathbb{R}: (a,b) \mapsto a-b$$
Now, because $\mathbb{Z}$ is closed in $\mathbb{R}$, and $f$ is continuous (as $(\mathbb{R},+)$ is a topological group), $f^{-1}(\mathbb{Z})$ must be closed as well.
In general we have: if $X$ is a topological group that is hausdorff, and $Y$ is a closed normal subgroup, then $X/Y$ is hausdorff