It seems to not be generally true that a subset $U$ of a simply connected space $E$, is simply connected. The most obvious example perhaps is that $\mathbb{R}^2$ is simply connected, but that $\mathbb{R}^2 \setminus\{(0,0)\}$ is not simply connected (take any loop going around $(0,0)$).
Are there further assumptions one can impose on either $E$ (e.g. locally path-connected/connected) and/or $U$ under (e.g. component/path-component of $E$) under which one can say that $U$ will be simply connected?