Fixing the values of the $ \lambda $s, write $ f ( x _ 1 , \ldots , x _ n ) $ for the left-hand side and $ g ( x _ 1 , \ldots , x _ n ) $ for the right-hand side (although this value of $ g $ happens to depend only on $ x _ 1 $ and $ x _ n $). Because both expressions may be written using only (finitely many) additions, multiplications, and divisions (by nonzero values), these are both continuous functions (on $ ( 0 , \infty ) ^ n $ rather than on $ \mathbb R ^ n $ to avoid division by zero). Now, $ g - f \geq 0 $ on the open set $ U = \{ x _ 1 , \ldots , x _ n \; | \; x _ 1 < \cdots < x _ n \} $, and the closure of this set (in $ ( 0 , \infty ) ^ n $) is $ \overline U = \{ x _ 1 , \ldots , x _ n \; | \; x _ 1 \leq \cdots \leq x _ n \} $. In other words, $ ( g - f ) [ U ] \subseteq [ 0 , \infty ) $; so $ ( g - f ) [ \overline U ] \subseteq \operatorname { Cl } [ 0 , \infty ) = [ 0 , \infty ) $. That is, $ g - f \geq 0 $ on $ \overline U $, which is what you want.
Short version: If a weak inequality between continuous functions holds assuming some conditions given by strict (or weak) inequalities (or equations), then it will continue to hold if all of the inequalities in the hypothesis are weakened (as long as everything is still continuous there). (Also, if a strict inequality holds under such conditions, then the weakening of the conclusion will hold if all of the inequalities in the hypothesis are weakened, in the same way.)
Note that we can't replace $ 0 < x _ 1 $ by $ 0 \leq x _ 1 $, since then the expressions could be undefined. (However you could interpret both sides as $ \infty $ when $ x _ 1 = 0 $ and then the inequality would continue to hold.)